The authors investigated how environmental factors (pH, temperature, incubation time and concentration of pollutants) effected three NHCs degrading strains’ biofilm forming. The results show significant effects of pH, temperature and incubation time on biofilm formation. The optimum environmental conditions for forming the maximum amount of biofilm is: pH=7, temperature 35°C, and incubation time 36 hours for BC026; pH=8, temperature 35°C, and incubation time 48 hours for BW001; and pH=7–9, temperature 40°C, and incubation time 36 hours for BW004. There is no significant impact on biofilm formation of any degrading bacterium exposed to 0–1600 mg/L of pollutant concentration.